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DEDICATION 1
DEDICATION 2
CERTIFICATE 1
CERTIFICATE-2
DECLARATION
ACKNOWLEDGEMENT
CONTENTS
LIST OF FIGURES
LIST OF PLATES
ABBREVIATIONS
I. INTRODUCTION
KERALA: LAKES, BACKWATERS AND RIVERS
AREA OF STUDY
RIVERS OF KERALA
LAKES AND BACKWATERS
The River Meenachil
II. REVIEW OF LITERATURE
PART I
II.1.1 Intestinal bacterial flora of avian fauna
II.1.2 Intestinal bacterial flora of Homo sapiens
PART II
II.2.1 Antibiotic sensitivity
II.3.1 Haemagglutination and haemolysis
II.4.1 Bacteriocin production
PART III
II.5.1 Effect of crop protection chemicals on microbial flora
II.6.1 Bacteriological studies on water
II.7.1 Survival ability of bacteria in aquatic environments
III. MATERIALS AND EXPERIMENTAL METHODS
PART I
III.1 Avian fauna subjected to investigation
III.1.1 Corvus splendens protegatus (Madarasz)
III.1.2 Phalacrocorax niger (Vieiilot)
III.1.3 Bubulcus ibis coromandus (Boddaert)
III.1.4 Anus crecca crecca (Linnaeus)
III.1.5 Anus domesticus (Domestic Duck)
III.1.6 Callus domesticus (Domestic Fowl)
III.1.7 Columba livia intermedia (Strickland)
Plate 1. Corvus splendens protegatus (Madarasz) The Common House Crow (Locality: Kottayam)
Plate 2. Corvus splendens protegatus (Madarasz) The Common House Crow (Locality: Ettumanoor)
Plate 3. Phalacrocorax niger (Vieillot) Little Cormorant (Locality: Kumarakom Tourist Complex)
Plate 4. Phalacrocorax niger (Vieillot) Cormorants and Darters (Locality: Kumarakom Tourist Complex)
Plate 5. Bubulcus ibis coromandus (Boddaert) Cattle Egret (Locality: Paddy fields, Athirampuzha)
Plate 6. Anas crecca crecca (Linnaeus) Common Teal (Locality: Kumarakom - Vembanad Lake)
Plate 7. Anas querquedula (Linnaeus) Garganey Teal (Locality: Kumarakom - Vembanad Lake)
Plate 8. Anas domesticus (Domestic Duck) (Locality: Athirampuzha)
Plate 9. Gallus domesticus (Domestic Fowl) (Locality: Poultry Farm, Kottayam)
Plate 10. Columba livia intermedia (Strickland) Indian Blue Rock Pigeon (Locality: Kottayam)
III.1.8 Collection of dropping pilings of birds
III.1.9 Isolation of bacteria from faecal samples of avian fauna
III.1.10 Identification of Bacterial Isolates
III.2 Antimicrobial Sensitivity Tests
III.2.1 Antimicrobial Drugs and Disc Potency Used for the Present Study
III.2.2 Stokes Method
III.3A Haemolysis
III.3B Haemagglutination Reaction
III.4 Test for Bacteriocin Production
III.5 Effect of Pesticides on Soil Microorganisms
III.6 Bacteriological Studies on Water Samples
III.7 Survival of Coliforms in Water Samples
IV. RESULTS
PART I
INTESTINAL GRAM NEGATIVE BACTERIAL FLORA OF AVIAN FAUNA
IV. 1.1 Incidence of bacterial species from avian faecal sources
Fig. 1 Percentage occurrence of Gram negative intestinal bacteria of avian fauna
Fig. 2. Percentage occurrence of Gram negative intestinal bacteria of Crow, Egret, Duck, and Cormorant
Fig. 3. Percentage occurrence of Gram negative intestinal bacteria of Teal, Pigeon, Fowl, and Cormorant
Plate 11. Cormorant: Direct smear from droppings (Gram stained)
Plate 12. Cormorant: Sweep smear from culture (Gram stained)
Plate 13. Crow: Sweep smear from culture (Gram stained)
Plate 14. Egret: Sweep smear from culture (Gram stained)
Plate 15. Teal: Direct smear from droppings (Gram stained)
Plate 16. Teal: Sweep smear from culture (Gram stained)
Plate 17. Duck: Sweep smear from culture (Gram stained)
Plate 18. Fowl: Sweep smear from culture (Gram stained)
Plate 19. Pigeon: Direct smear from droppings (Gram stained)
Plate 20. Pigeon: Sweep smear from culture (Gram stained)
PART II
Characterisation
IV.2.1 Antimicrobial susceptibility profiles of bacterial isolates from human and avian sources
IV.2.2 Multiple drug resistance of bacterial isolates from human and avian sources
Fig. 4. Antibiotic susceptibility profiles of E. coli from avian and human sources
Fig. 5. Antibiotic susceptibility profiles of Klebsiella sp. from avian and human sources.
IV.3A.1 Haemolytic property of the human and avian Gram negative bacterial isolates
IV. 3B.1 Haemagglutination activity of the human and avian Gram negative bacterial isolates
IV.4.1 Bacteriocin production profile of Gram negative enteric bacteria from human and avian sources
Fig. 6. Incidence of drug resistance in bacteriocinogenic and nonbacteriocinogenic strains - avian source
Fig. 7. Incidence of drug resistance in bacteriocinogenic and nonbacteriocinogenic strains - human source
Plate 21. Bacteriocin production (Negative.)
Plate 22. Bacteriocin production (Positive)
PART III
COLIFORMS AND OTHER ENVIRONMENTAL BACTERIAL FLORA
I V.5.1 Effect of crop protection chemicals on bacterial flora of the soil
IV.6.1 Bacteriological studies on water-prevalence of coli forms
IV.6.2 Eijkman test and faecal coliforms
1V.6.3 Incidence of Gram positive bacteria in various aquatic environmtents
IV.6.4 Incidence of NLF bacteria in various aquatic environments
IV.6.5 E. coli count and environmental parameters
Fig. 8. Seasonal variations of bacteria in well water
Fig. 9. Seasonal variations of bacteria in pond water
Fig. 10. Seasonal variations of bacteria in paddy field water
Fig. 11. Seasonal variations of bacteria in streams
Fig. 12. Seasonal variations of bacteria in river water
IV.7.1 Survival ability of coliforms and Gram negative pathogenic enteric bacteria in various aquatic environments
Fig. 13. Average survival ability of bacteria in various aquatic environments
Fig. 14. Relationship between survival rate and salinity percentage of E. coli in freshwaters.
Fig. 15. Relationship between survival rate and organic content of E. coli in freshwaters
V. DISCUSSION
PART I
V.1.1 Prevalence of bacterial species from avian faecal sources.
Plate 23. Himantopus himantopus himantopus (Blackwinged stilt) (Locality: Athirampuzha)
Plate 24. Kumarakom backwaters
PART II
V.2.1 Antimicrobial susceptibility profiles
V.3A.1 Haemolysin production activity of bacterial isolates from human and avian sources
V.3B.1 Haemagglutination activity of the human and avian isolates.
V.4.1 Bacteriocin production profile of Gram negative enteric bacteria from human and avian sources
PART III
V.5.1 Effect of pesticides on microbial flora of the environment.
V.6.1 Bacteriological studies on samples from freshwater environments
V.7.1 Survival ability of Gram negative bacteria in aquatic environments
VI. SUMMARY AND CONCLUSION
BIBLIOGRAPHY
APPENDIX